5.4.99.61 | apo form and complexed with hydrogenobirinate, hanging drop vapor diffusion with equal volumes of concentrated protein, i.e. 10 mg/ml, and a precipitant solution containing 25%-32.5% polyethylene glycol 4000, 200 mM MgCl2, 100 mM Tris-HCl, pH 8.5, crystals diffract to 2.1 A and 2.6 A resolution |