EC Number   |
|---|
   5.4.99.25 | atomic-resolution crystal structure, TruB bound to RNA at 1.85 A resolution |
| 5.4.99.25 | crystal structure is diffracted to a resolution of 1.7 A. The crystal belong to space group P2, with unit-cell parameters a = 37.65, b = 78.09, c = 56.33 A , c = 102.05 |
| 5.4.99.25 | crystal structure of the Y67F mutant in complex with a 5-fluorouridine-tRNA at 2.4 A resolution |
| 5.4.99.25 | sitting drop vapor diffusion method, structure of pseudouridine55 tRNA in two substrate-free forms, at 1.9 A resolution |
| 5.4.99.25 | the TruB crystallization construct (tTruB), differs from full-length Escherichia coli TruB in having an N-terminal His-tag followed by residue 10 of the full-length protein. tTruB(D48N) is cocrystallized with [5-fluorouridine]-RNA. Initially, crystals are obtained by seeding with fragments of tTruBRNA cocrystals. The resulting crystals are crushed and used as seeds for six subsequent sequential rounds of seeding into tTruB(D48N)RNA complex crystallization experiments |
| 5.4.99.25 | vapor-phase diffusion method, crystal structure of TruB apoenzyme in the absence of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound forms of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity |
| 5.4.99.25 | vapor-phase diffusion method, crystal structure of TruB in complex with 17-base stem-loop of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound form of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity |
