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Results 1 - 3 of 3
EC Number Crystallization (Commentary) Reference
Show all pathways known for 5.2.1.1Display the word mapDisplay the reaction diagram Show all sequences 5.2.1.1- 2451
Show all pathways known for 5.2.1.1Display the word mapDisplay the reaction diagram Show all sequences 5.2.1.1im complex with maleate 715258
Show all pathways known for 5.2.1.1Display the word mapDisplay the reaction diagram Show all sequences 5.2.1.1purified recombinant wild-type alone, and mutant enzyme C200A in complex with substrate maleate, hanging-drop vapour-diffusion method, mixing of 15-20 mg/ml protein in 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 2 mM DTT, with a reservoir solution containing 25% w/v PEG3,350 and 0.1 M Tris, pH 8.5, for the substrate-enzyme mutant complex, and 1.4 M (NH4)2SO4, 120 mM NaCl, and 0.1 M HEPES, pH 7.5, for the free wild-type enzyme, 14°C, X-ray diffraction structure determination and analysis at 2.95 A and 2.10 A resolution, respectively 728334
Results 1 - 3 of 3