EC Number   |
Reference |
|---|
    5.1.3.1 | crystal structure of the RPE is solved at 1.8 A resolution in the presence of D-xylitol 5-phosphate, an inert analogue of the D-xylulose 5-phosphate substrate. This structure suggests that the 2,3-enediolate intermediate in the 1,1-proton transfer reaction is stabilized by bidentate coordination to the Zn2+ that also is liganded to His 34, Asp 36, His 67, and Asp 176, the carboxylate groups of the Asp residues are positioned also to function as the acid/base catalysts |
672127 |
| 5.1.3.1 | crystals are grown using the microbatch technique from 22% methyl ether PEG 2000, 200 mM MgCl2, 100 mM MES pH 6.0. The crystals belong tp space group P2(1), with unit-cell parameters a = 82.6 A, b = 87.8 A, c = 97.8 A, beta = 114.6°, 1.6 A resolution |
660588 |
| 5.1.3.1 | crystals are produced with hanging drop method using 6 mg/ml cytosolic enzyme, 1.0 M ammonium sulfate, 50 mM Mops, pH 7.5, 1% w/v polyethylene glycol 400, 10 mM glycerol-3-phosphate and 5 mM L-arginine in the drop |
652903 |
| 5.1.3.1 | hanging drop vapor diffusion method |
652793 |
| 5.1.3.1 | hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 100 mM Bis-Tris (pH 5.5), and 200 mM NaCl |
714945 |
