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EC Number	Crystallization (Commentary)
5.1.2.1	purified enzyme, free or Hg-labeled, hanging drop vapour diffusion method, mixing of 0.002 ml of 16 mg/ml protein solution with 0.002 ml of reservoir solution containing 22% PEG monomethyl ether 5000, 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 0.2 M sodium malonate pH 7.0 and 0.02% w/v sodium azide, at 18°C, a few days, X-ray diffraction structure determination and analysis at 1.8-2.0 A resolution

EC Number

Crystallization (Commentary)

5.1.2.1

purified enzyme, free or Hg-labeled, hanging drop vapour diffusion method, mixing of 0.002 ml of 16 mg/ml protein solution with 0.002 ml of reservoir solution containing 22% PEG monomethyl ether 5000, 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 0.2 M sodium malonate pH 7.0 and 0.02% w/v sodium azide, at 18°C, a few days, X-ray diffraction structure determination and analysis at 1.8-2.0 A resolution

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Release 2023.1 (January 2023)