EC Number   |
Reference |
|---|
    5.1.1.13 | catalytically inactive mutant C82A in complex with citric acid, 2.0 A resolution. Citric acid binds to the catalytic site, which induces a conformational change to close the active site. Residue R48 is responsible for recognizing carboxyl groups of the substrates L-/D-aspartates and stabilizing a reaction intermediate, and L164 is responsible for stabilizing a closed state structure |
695052 |
| 5.1.1.13 | purified enzyme, sitting drop vapour diffusion method with microseeding, mixing of 0.002 ml of 20 mg/ml protein in 50 mM MES, pH 6.5, and 4 mM dithiothreitol, with 0.002 ml of reservoir solution containing 25% v/v PEG MME 550, and 5% v/v 2-propanol, and 0.1 M sodium acetate, pH 4.8, equilibration againat 0.1 ml reservoir solution, 20°C, single rod-shaped crystals, X-ray diffraction structure determination and analysis at 2.55 A resolution, molecular replacement calculations are carried out using the coordinates of PhAspR, PDB ID 1jfl, as a starting model |
746649 |
| 5.1.1.13 | purified recombinant enzyme, vapour diffusion method, mixing of 8-12 mg/ml protein in 50 mM Tris-HCl, pH 8.0, with reservoir solution containing 20-25% w/v PEG 3400, 200 mM potassium/sodium tartrate, and 1 mM L-aspartate, X-ray diffraction structure determination and analysis at 1.85 A resolution, molecular replacement method using the structure of aspartate racemase from Salmonella typhimurium, PDB ID 3S81, as a probe model |
747720 |
| 5.1.1.13 | purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.006 ml of 2.5 mg/ml protein in 10 mM Tris-HCl, 20 mM pyridoxal 5'-phosphate, 1 mM 2-mercaptoethanol, pH 8.8, with 0.003 ml of reservoir solution containing 27% w/v PEG 4000, 50 mM sodium acetate, pH 5.0, 200 mM ammonium acetate, and 15% v/v glycerol, and equilibration against 0.1 ml of reservoir solution, 14°C, X-ray diffraction structure determination and analysis at 1.90 A resolution |
746669 |
| 5.1.1.13 | sitting drop vapour diffusion method at 293 K, space group P21 with a: 65.5 A, b: 58.7 A, c: 67 A and beta 109.6° |
652886 |
| 5.1.1.13 | sitting drop vapour diffusion method at 293 K, space groups: P21 with a: 65.5 A, b: 58.7 A, c: 67.0 A, and beta: 109.6°, P212121 with a: 68.1 A, b: 135 A and c: 151.5 A, and P3121 with a and b: 80.6 A and c: 70.3 A |
649148 |
