EC Number   |
Reference |
|---|
    4.2.3.4 | - |
137643 |
| 4.2.3.4 | by using the hanging-drop vapour-diffusion method with different screening kits, yields crystals belonging to the tetragonal space group, crystals of DHQS obtained in sitting-drop setups using a hydra automated high-throughput crystal screening machine with different screening kits: (a) 0.1 M sodium malonate pH 4.0, 12% PEG 3350, (b) 0.2 M sodium malonate pH 6.0, 20% PEG 3350, (c) 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate, (d) 0.15 M caesium chloride, 15% PEG 3350, (e) 1.0 M sodium citrate, 0.1 M CHES pH 9.5, (f) 0.2 M ammonium sulfate, 0.1 M bis-Tris pH 5.5, 25% PEG 3350, (g) 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 30% PEG MME 5000, (h) 0.02 M zinc chloride, 20% PEG 3350. Best crystals are obtained after two weeks from a reservoir containing 0.2 M ammonium sulfate, 0.1 M MES monohydrate pH 6.0 and 31%PEG MME 5000 |
690254 |
| 4.2.3.4 | molecular replacement based on the structure of Aspergillus nidulans enzyme, PDB code 1NR5, revealing a homodimeric protein. Each monomer has a NAD+ binding site nestled between the distinct N- and C-terminal domains |
729105 |
| 4.2.3.4 | obtained in a solution containing 20 mM NAD+ |
690739 |
| 4.2.3.4 | oil microbatch method, homodimer 1.8 A resolution |
666871 |
| 4.2.3.4 | sitting-drop vapour-diffusion crystallisation at 4°C utilising microbridges, crystals of unliganded enzyme as well as in complexes with NAD+, the substrate analogue [1R-(1alpha,3beta,4alpha,5beta)]-5-phosphonomethyl-1,3,4-trihydroxycyclohexane-1-carboxylic acid and together with both, NAD+ and [1R-(1alpha,3beta,4alpha,5beta)]-5-phosphonomethyl-1,3,4-trihydroxycyclohexane-1-carboxylic acid |
666031 |
| 4.2.3.4 | sitting-drop vapour-diffusion crystallisation at 4°C utilising microbridges, crystals of unliganded enzyme as well as in complexes with NAD, the substrate analogue [1R-(1alpha,3beta,4alpha,5beta)]-5-phosphonomethyl-1,3,4-trihydroxycyclohexane-1-carboxylic acid and together with both, NAD and [1R-(1alpha,3beta,4alpha,5beta)]-5-phosphonomethyl-1,3,4-trihydroxycyclohexane-1-carboxylic acid |
666031 |
| 4.2.3.4 | sitting-drop vapour-diffusion, crystals of unliganded enzyme, binary complexes with either the substrate analogue, carbaphosphonate or the cofactor NADH, as well as the ternary enzyme-carbaphosphonate-cofactor complex |
649156 |
| 4.2.3.4 | sitting-drop vapour-diffusion, structure at 1.7 A resolution |
649245 |
| 4.2.3.4 | structure in complex with NAD and with NAD plus chlorogenic acid. Chlorogenic acid occupies the substrate position and coordinates with the metal ion in the substrate-binding pocket |
763209 |
