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EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.118in complex with substrate analogues geranyl-S-thiolodiphosphate and 2-fluorogeranyl diphosphate at 1.80 and 1.95 A resolution, respectively. MIBS adopts the tertiary structure of a class I terpenoid cyclase. The dimeric quaternary structure is different, the active sites in the MIBS dimer are oriented in parallel fashion and the dimer interface in MIBS is formed by helices D1, D2
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.118in unliganded state and in complex with two Mg2+ ions and 2-fluoroneryl diphosphate at 1.85 and 2.00 A resolution, respectively. Cocrystallization with the fluorinated analogue of the tertiary allylic diphosphate intermediate, 2-fluorolinalyl diphosphate yields the crystal structure of the complex with the primary allylic diphosphate, 2-fluoroneryl diphosphate. The crystalline enzyme active site remains partially open, presumably due to the binding of only two Mg2+ ions
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