4.2.3.114 | purified recombinant enzyme, sitting drop method, mixing of 400-600 nl of 10 mg/ml protein in 20 mM Tris-HCl, pH 7.8, 1 mM MgCl2, 4 mM DTT, with reservoir solution containing 0.2 M ammonium tartrate, pH 7.0, 20% w/v PEG 3350, in a 1:1 or 1:2 ratio, equilibration against 0.07 ml reservoir solution, 19°C, X-ray diffraction structure determination and analysis at 1.65-1.90 A resolution, two molecules (monomers A and B) in the asymmetric unit, molecular replacement using the crystal structure of (4S)-limonene synthase from Mentha spicata, PDB ID 2ong, model building |
746657 |