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EC Number	Crystallization (Commentary)
4.2.1.163	hanging drop vapor diffusion method, crystals of the native and selenomethionine-labelled enzyme are grown at 0.1M MES (pH 6.0), 0.7 M potassium thiocyanate, 7% (w/v) PEG 12000, 5% (w/v) PEG 550 MME, 10 mM dithiothreitol and 0.1 M Tris (pH 7.0), 0.7 M potassium thiocyanate, 6.4% (w/v) PEG 20000, 7% (w/v) PEG 550 MME,10 mM dithiothreitol, respectively
4.2.1.163	sitting drop vapor diffusion method, using 6.4% (w/v) polyethylene glycol 20000, 5% (w/v) polyethylene glycol 550 monomethyl ether, 0.7 M potassium thiocyanate, in 50 mM MES pH 5.5

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Release 2023.1 (January 2023)