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EC Number Crystallization (Commentary)
Display the reaction diagram Show all sequences 4.2.1.158wild-type and mutant Y90F. A Tyr 164-Arg 162 dyad is the base that initiates the reaction by abstraction of the alpha-proton and Tyr 90 is the acid that facilitates departure of the beta-OH leaving group. The structure contains two Mg2+ ions located 10.4 A from one another, with one located in the canonical position in the (beta/alpha)7beta-barrel, the second is located in a site within the capping domain
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