EC Number   |
Reference |
|---|
   4.2.1.112 | high resolution crystal structure determination of the W-dependent enzyme crystallized under the exclusion of dioxygen (N2/H2 (94%/6% v/v)) at 1.26 A resolution, PDB ID 2E7Z |
748243, 748431 |
| 4.2.1.112 | purified enzyme, the mother liquor contains plus 15% (v/v) 2-methyl-2,5-pentanediol, X-ray diffraction structure determination and analysis at 1.26-1.95 A resolution, modeling |
670710 |
| 4.2.1.112 | purified native enzyme, sitting drop vapour diffusion method in a 95%N2/5%H2 atmosphere, 10 mg/ml protein in 5 mM HEPES-NaOH, pH 7.5, and 3 mM dithionite or Ti(III)citrate, mixing with an equal volume of 0.002 ml of precipitant solution equilibrated against 0.3 ml of reservoir, 20°C, 3 weeks, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement |
667090 |
| 4.2.1.112 | purified recombinant NarG-fusion acetylene hydratase, sitting and hanging drop vapor diffusion methods, small crystals after 3 to 4 weeks, 6.5-10 mg/ml protein in solution containing 5 mM HEPES-NaOH, pH 7.5, and 7.5 mM Na2S2O4, cryoprotection by 20% v/v 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis |
715374 |
| 4.2.1.112 | the enzyme complexed with the aldehyde 4-oxobutyl diphosphate, X-ray diffraction structure determination and anaysis at 1.8 A resolution |
730116 |
