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Results 1 - 4 of 4
EC Number Crystallization (Commentary)
Show all pathways known for 4.2.1.104Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.104-
Show all pathways known for 4.2.1.104Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.104enzyme SpCynS as DNA-protein complex is crystallized as an impurity and its identity is determined using mass-spectrometric analysis. The crystals seems to have a ligand bound in the active site, glycerol molecules are bound at the entry to the active site of the enzyme. Mixing of 500 nl of 5.5 mg/ml protein-DNA complex in 20 mM MES potassium, pH 6.5, 60 mM KCl, 5 mM MgCl2, 2 mM DTT, with 500 nl of reservoir solution containing 0.1 M HEPES, pH 7.5, 2% v/v 2-propanol, 0.1 M sodium acetate, 18% w/v PEG 8000, and equilibration against 0.055 ml of reservoir solution, four months at 20°C, X-ray diffraction structure determination and analysis at 2.1 A resolution, the cryoprotecting solution consists of mother liquor containing 25% v/v glycerol, the crystals belong to space group P1, molecular replacement solution using the target DNA-protein complex or its components as a search model and the EcCynS crystal structure (PDB ID 1dw9) as a search model, modeling without ligand
Show all pathways known for 4.2.1.104Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.104purified recombinant MBP-tagged enzyme, sitting drop vapor diffusion method, 13 mg/ml protein solution is mixed with crystallization solution, containing 0.1 M Tris, pH 7.5, 15% w/v PEG 6000, in a 1:1 ratio, about 2 months, room temperature, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacement using the crystal structure of Escherichia coli cyanase (PDB ID 2IV1) as the starting model
Show all pathways known for 4.2.1.104Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.104selenomethionine-labeled purified recombinant enzyme, also crystals of enzyme with complexed inhibitors oxalate and chloride, 2 mM dithiothreitol, sitting drop vapour diffusion method, from 2.1 M ammonium sulfate, 50 mM Na2KPO4, pH 7.3, microseeding with large sitting drops at 18°C with wild-type crystals, 5-7 days, X-ray diffraction structure determinzation and analysis at 1.65 A resolution by multiwavelength anomalous diffraction MAD method
Results 1 - 4 of 4