EC Number   |
Reference |
|---|
    4.2.1.10 | - |
5478, 5485, 653902 |
| 4.2.1.10 | 3 different crystal forms |
649195 |
| 4.2.1.10 | crystal structure in complex with (2R)-2-methyl-3-dehydroquinic acid, a substrate analogue, at 1.5 A. Residues Gln236, Pro234 and Ala233 are located in the flexible substrate-covering loop. Gln236 is responsible for the folding of this loop and for the dramatic reduction of its flexibility, which triggers active site closure. Glu46 is important in bringing the substrate close to the lysine/histidine catalytic pocket to initiate catalysis |
747022 |
| 4.2.1.10 | crystals obtained in space and on earth using the counter-diffusion technique |
649240 |
| 4.2.1.10 | DHQ2 in complex with (2R)-2-p-methoxybenzyl-3-dehydroquinic acid, sitting drop vapour diffusion method, 20 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 1 mM 2-mercaptoethanol, 1 mM EDTA, and 200 mM NaCl, with addition of (2R)-2-p-methoxybenzyl-3-dehydroquinic acid at 0.25 M in methanol and added at a ratio of 1:20 v/v, mixing of 0.002 ml protein solution with 0.002 ml reservoir solution containing 31% w/v PEG 4000 and 0.1 M sodium citrate, pH 5.0, and equilibation against 0.15 ml reservoir solution, room temperature, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution, modelling |
714749 |
| 4.2.1.10 | DHQ2 in complex with (2R)-2-p-methoxybenzyl-3-dehydroquinic acid, sitting drop vapour diffusion method, 20 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 1 mM 2-mercaptoethanol, 1 mM EDTA, and 200 mM NaCl, with addition of (2R)-2-p-methoxybenzyl-3-dehydroquinic acid at 0.25 M in methanol and added at a ratio of 1:20 v/v, mixing of 0.002 ml protein solution with 0.002 ml reservoir solution containing 32% v/v 2-methyl-2,4-pentanediol, 0.3 M ammonium sulfate and 0.1 M HEPES, pH 7.5, and equilibation against 0.15 ml reservoir solution, room temperature, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution, modelling |
714749 |
| 4.2.1.10 | DHQ2 in complex with inhibitors (4R,6R,7S)-4,6,7-trihydroxy-2-[(1Z)-prop-1-en-1-yl]-4,5,6,7-tetrahydro-1-benzothiophene-4-carboxylic acid and (4R,6R,7S)-2-(1-cyclopropylethyl)-4,6,7-trihydroxy-4,5,6,7-tetrahydro-1-benzothiophene-4-carboxylic acid, X-ray diffraction structure determination and analysis at 1.95 and 1.85 A resolution, respectively |
714752 |
| 4.2.1.10 | DHQase-AH9095 complex |
677327 |
| 4.2.1.10 | enzyme in complex with product 3-dehydroshikimate, X-ray diffraction structure determination and analysis |
714105 |
| 4.2.1.10 | hanging drop vapor diffusion method |
649154, 651312 |
