EC Number   |
Reference |
|---|
   4.1.99.14 | purified recombinant His6-tagged mutant enzyme Y98F, with reconstituted iron-sulfur cluster, hanging drop vapor diffusion method, using 70 mM octanoyl-N-hydroxyethylglucamide, 200 mM lithium sulfate, 100 mM Tris-HCl, pH 9.0 and 19-27% w/v PEG 8000, X-ray diffraction structure determination and analysis |
727020 |
| 4.1.99.14 | purified recombinant reconstituted C140A/S76C mutant SP lyase, hanging drop vapor diffusion method, the enzyme is incubated with Se-SAM, crystallization in the presence of 70 mM octanoyl-N-hydroxyethylglucamide and mixing with a reservoir solution containing 200 mM lithium sulfate, 100 mM Tris-HCl, pH 9.0, and 19-27% w/v PEG 8000 under anaerobic conditions, 20°C, X-ray diffraction structure determination and analysis at 2.1-2.6 A resolution |
747506 |
| 4.1.99.14 | purified wild-type and mutant enzymes in complex with the S-adenosyl-L-methionine cofactor and the [4Fe-4S] cluster, hanging drop vapor diffusion method, using 70 mM octanoyl-N-hydroxyethylglucamide and a reservoir solution containing 200 mM lithium sulfate, 100 mM Tris-HCl, pH 9.0, and 19-27% w/v PEG 8000., 20°C, cryoprotection of crystals by 3 mM dithiothreithol, 500 mM NaCl, and 15% v/v ethylene glycol in mother liquor, X-ray diffraction structure determination and analysis at 2.0-2.1 A resolution |
728401 |
