EC Number |
Reference |
---|
4.1.99.1 | - |
37346, 37347, 37349, 37360, 37365 |
4.1.99.1 | analysis of the enzyme tetramer with one pyridoxal 5'-phosphate bound to each monomer, crystal structure at 2.1 A resolution, PDB ID 1AX4 |
747245 |
4.1.99.1 | apo-form of enzyme without bound pyridoxal 5-phosphate but with two bound sulfate ions, hanging drop vapor diffusion method |
671054 |
4.1.99.1 | apo-form of the enzyme, hanging drop vapor diffusion method |
677332 |
4.1.99.1 | crystallized in the apo form by the hanging-drop vapour-diffusion method using polyethylene glycol 400 as a precipitant and magnesium chloride as an additive. The crystals belong to the orthorhombic space group F222, with unit-cell parameters a = 118.4 A, b = 120.1 A, c = 171.2 A. Contains a monomer in the asymmetric unit with a solvent content of 55%. Tryptophanase mutants W330F and Y74F are crystallized under the same conditions and the crystals diffracted to a resolution limit of 1.9 A |
663488 |
4.1.99.1 | in complex with oxindolyl-L-alanine and with L-tryptophan and L-serine |
652265 |
4.1.99.1 | mutant enzymes Y74F and C298S, hanging drop vapor diffusion method, using 30% (w/v) PEG 400, 100 mM HEPES pH 7.5, 200 mM MgCl2, 5 mM 2-mercaptoethanol |
702931 |
4.1.99.1 | purified recombinant apo-enzyme in an open conformation, hanging drop vapour diffusion method with 25-30% PEG 4000, 30 mM ammonium sulfate, 20 mM 2-mercaptoethanol, 100 mM Tris-HCl, pH 9.0, X-ray diffraction structur determination and analysis at 2.8 A resolution |
746651 |
4.1.99.1 | purified recombinant enzyme in complex with inhibitor oxindolyl-L-alanine (OIA), hanging-drop vapor diffusion method by mixing 15 mg/ml protein with an equal volume of reservoir solution consisting of 35 mM potassium phosphate, 0.05 M HEPES, pH 7.0, 0.3 M KCl, and 11% PEG 4000, at 20°C, the crystals are transferred to reservoir solution supplemented with 20% 1:1:1 ethylene glycol:DMSO:glycerol as cryosolvent, with or without 10 mM OIA, X-ray diffraction structure determination and analysis at 2.0-2.1 A resolution |
746632 |
4.1.99.1 | purified recombinant tryptophanase in holo- and semi-holoforms, hanging drop vapour diffusion method, mixing of 15-25 mg/ml protein in 50 mM sodium phosphate, pH 6.0, 5 mM 2-mercaptoethanol, and 0.5-2 mM pyridoxal 5'-phosphate in a ratio of 3:1 with precipitant solution containing 10-15% w/v ammonium sulfate, 25 mM potassium acetate, pH 5.4, and 3-10 mM2-mercaptoethanol, 2-7 days, X-ray diffraction structure determination and analysis at 2.9-3.2 A resolution, both crystal forms belong to the same space group P43212 but have slightly different unit-cell parameters, molecular replacement using the structure of apo Trpase, PDB ID 2oqx, as the search model |
746654 |