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EC Number Crystallization (Commentary)
Show all pathways known for 4.1.3.30Display the word mapDisplay the reaction diagram Show all sequences 4.1.3.30crystals of the inactive mutant C123S MICL in complex with isocitrate are obtained at room temperature by vapor diffusion in hanging drops. A crystal of C123S MICL in complex with pyruvate and succinate was obtained by overnight incubation of a C123S MICL/isocitrate complex crystal in a solution containing 35% PEG3350, 100 mM pyruvate and 100 mM succinate. This crystal also belongs to space group C2
Show all pathways known for 4.1.3.30Display the word mapDisplay the reaction diagram Show all sequences 4.1.3.30native enzyme and bound with pyruvate/Mg2+, sitting and hanging drop vapor diffusion method
Show all pathways known for 4.1.3.30Display the word mapDisplay the reaction diagram Show all sequences 4.1.3.30sitting drop vapor diffusion method
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