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EC Number Crystallization (Commentary) Reference
Show all pathways known for 4.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.25purified His-tagged enzyme as apoenzyme and in complex with pyridoxal 5'-phosphate (PLP), sitting drop vapor diffusion method, mixing of 0.002 ml of 10 mg/ml protein solution with 0.002 ml of reservoir solution containing 0.1 M sodium cacodylate trihydrate, pH 7.5, 0.2 M MgCl2, and 18% PEG 1000, for the SeMet-LbTDC enzyme variant 0.1 M sodium cacodylate trihydrate, pH 7.0, 0.1 M MgCl2, and 16% PEG 4000 is used, the LbTDC-PLP complex is also crystallized at a molar ratio of 1:4 under the same conditions as SeMet-LbTDC, 2-5 days, 18°C, X-ray diffraction structure determination and analysis at 1.73-1.90 A resolution 749322
Show all pathways known for 4.1.1.25Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.25purified recombinant tagged enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 25 mM HEPES, pH 7.0, and 0.04 mM pyridoxal 5'-phosphate, with 0.001 ml of reservoir solution containing 0.2 M NaOAc and 1 M NH4H2PO4, and equilibration against 0.45 ml of reservoir solution, X-ray diffraction structure determination and analysis at 3.1 A resolution 747859
Results 1 - 2 of 2