   3.7.1.18 | OCH mutant H122A in complex with the minor diastereoisomer of (2S,4S)-alpha-campholinic acid, vapor diffusion hanging drop technique, mxing of protein solution containing 10 mg/ml protein in 50 mM Tris-HCl, pH 7.1, 1 mM dithiothreitol, and 0.02 mM phenylmethylsulfonyl fluoride with reservoir solution containing 0.1 M 2-(N-morpholino)ethanesulfonic acid, pH 5.6, 0.2 M calcium acetate, and 26% v/v PEG monomethyl ether 2000, in a 1:1 ratio, soakong of crystals in reservoir solution containing 6-oxocamphor for 30 min, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement |
719819 |
| 3.7.1.18 | selenomethionine-labeled enzyme, vapor diffusion hanging drop technique, protein solution containing 10 mg/ml protein in 50 mM Tris-HCl, pH 7.0, 1 mM dithiothreitol, 0.020 mM phenylmethylsulfonyl fluoride is mixed in a 1:1 ratio with reservoir solution, that contains for the native enzyme, the reservoir solution consisted of 0.1 M sodium acetate buffer, pH 4.5, 0.2 M ammonium sulfate, and 28% v/v PEG 4000, and for the selenomethionyl enzyme 0.1 M MES, pH 5.5, 0.2 M ammonium sulfate, 37.5% v/v 5,000 Da monomethyl ether, and 0.2% w/v n-octyl-beta-D-glucopyranoside, X-ray diffraction structure determination and analysis at 2.0-2.4 A resolution, selenomethionine multiple wave anomalous dispersion, molecular modeling |
719814 |
