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EC Number Crystallization (Commentary)
Display the reaction diagram Show all sequences 3.7.1.11crystal structure analysis of CDH in complex with substrate cyclohexane-1,2-dione, PDB ID 2PGN
Display the reaction diagram Show all sequences 3.7.1.11purified CDH from strain 22Lin, hanging drop vapour diffusion method, equal volumes of protein storage and reservoir solution, the latter composed of 60% MPD, 20 mM Na+ acetate and 200 mM NaCl, 25°C, a second cubic crystal form is obtained at 20°C with a reservoir solution containing 17-18% w/v PEG 8000, 100 mM Na+ acetate, 10 mM MnCl2, and 2% w/v isopropanol in 0.1 M HEPES buffer, pH 7.5, X-ray diffraction structure determination and analysis at 1.26 A resolution, modeling
Results 1 - 2 of 2