3.7.1.11 | purified CDH from strain 22Lin, hanging drop vapour diffusion method, equal volumes of protein storage and reservoir solution, the latter composed of 60% MPD, 20 mM Na+ acetate and 200 mM NaCl, 25°C, a second cubic crystal form is obtained at 20°C with a reservoir solution containing 17-18% w/v PEG 8000, 100 mM Na+ acetate, 10 mM MnCl2, and 2% w/v isopropanol in 0.1 M HEPES buffer, pH 7.5, X-ray diffraction structure determination and analysis at 1.26 A resolution, modeling |