EC Number   |
|---|
    3.5.99.6 | - |
| 3.5.99.6 | isozyme GNPDA1, X-ray diffraction structure determination and analysis at 1.75 A resolution |
| 3.5.99.6 | purified recombinant His-tagged enzyme, the protein solution contains 10 mg/ml protein in 20 mM Tris-HCl, 150 mM NaCl, pH 7.5, mixing of 0.001 ml of protein and 0.001 ml of reservoir solution, and equilibration against 0.5 ml of reservoir solution, hanging drop vapour diffusion method, X-ray diffraction structure determination and anaylsis at 2.4 A resolution |
| 3.5.99.6 | structure of the enzyme in complexes with its allosteric activator and with a competitive inhibitor |
| 3.5.99.6 | the crystallographic structure of F174A is determinated |
| 3.5.99.6 | the structure of five complexes of the enzyme in R and T state is described and their kinetic and allosteric implication analysed, the ligand-free enzyme, T-conformer, undergoes an allosteric transition to the free active-site R conformer upon binding of the allosteric activator |
