3.5.2.5 | purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml 14 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 20% w/v PEG 3350, and 0.1 M potassium thiocyanate, pH 6.5, equilibration against 0.5 ml reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 3.5 A resolution |