EC Number   |
Reference |
|---|
    3.5.1.9 | enzyme in complex with inhibitor PMSF, X-ray diffraction structure determination and analysis, molecular replacement using structure PDB ID 2PBL |
733287 |
| 3.5.1.9 | purified detagged recombinant enzyme, hanging drop vapour diffusion method, mixing of 4.0 mg/ml protein in 20 mM Tris-HCl, pH 7.4, and 200 mM NaCl, and 8 mM kynurenine, in a 1.1 ratio with reservoir solution containing 100 mM Tris-HCl, pH 8.5, 150 mM MgCl2, 30% w/v PEG 4000 and 1.5% v/v dioxane, final volume 0.002-0.004 ml, 20°C, 5 days, X-ray diffraction structure determination and analysis at 1.95 A resolution |
733300 |
| 3.5.1.9 | purified detagged recombinant enzyme, sitting drop vapour diffusion method, mixing of 7.5 mg/ml protein in 25 mM Tris-HCl, pH 7.5, and 100 mM NaCl, and kynurenine, in a 1.1 ratio with reservoir solution containing 0.1M HEPES, pH 7.5, 20% w/v PEG 4000 and 10% v/v propan-2-ol, final volume 0.002-0.004 ml, 5 days at room temperature, X-ray diffraction structure determination and analysis at 2.37 A resolution, molecular replacement |
733300 |
| 3.5.1.9 | purified detagged recombinant enzyme, sitting drop vapour diffusion method, mixing of 7.5 mg/ml protein in 25 mM Tris-HCl, pH 7.5, and 100 mM NaCl, and kynurenine, in a 1.1 ratio with reservoir solution containing 10-16% w/v PEG 3350, 5 mM CoCl2, 5 mM CdCl2, 5 mM MgCl2 and 5 mM NiCl2, final volume 0.002-0.004 ml, 5 days at room temperature, X-ray diffraction structure determination and analysis at 1.60 A resolution |
733300 |
