3.5.1.62 | purified enzyme in complex with 5 different inhibitors, sitting drop vapor diffusion method, mixing of 500 nl of 5-10 mg/ml of protein in 20 mM Tris, pH 8.0, 50 mM NaCl, 0.01% sodium azide, 0.5-2 mM ZnCl2, and 2.5 mM inhibitor, with 500 nl of precipitant solution containing 0.2 M LiNO3, or KNO3, or LiCL, and 20% w/v PEG 3350, and equilibration against 0.1 ml of precipitant solution reservoir, 2 days, 21°C, X-ray diffraction structure determination and analysis at 1.13-1.42 A resolution |