3.5.1.48 | purified enzyme zHDAC10 mutant Y307F complexed with a polyamine transition state analogue inhibitor AAT, sitting drop vapour diffusion method, mixing of 400 nl of 30 mg/ml Y307F zHDAC10DELTA-AAT complex and 5 mM AAT with 400 nl of precipitant solution containing 0.2 M KH2PO4, and 20% PEG 3350, and equilibration against 0.08 ml precipitant solution, 24 h, 4°C, X-ray diffraction structure determination and analysis at 2.85 A resolution, molecular replacement using structure of the zHDAC6 CD1-TSA complex (PDB ID 5EEF) |