   3.4.22.59 | recombinant caspase-6 comprising residues 24179 and 194293 after self-cleavage of the zymogen, overall structure of the four caspase-6 p202/p102 tetramers in the asymmetric unit, and caspase-6 in complex with inhibitor Ac-VEID-CHO, hanging-drop vapor diffusion method, mixing of 0.001 ml protein solution containing 20 mM sodium acetate, pH 5.5, 50 mM NaCl, and 0.5 mM Tris(hydroxypropyl)phosphine with 0.001 ml of reservoir solution consisting of 3.3 M sodium nitrate, 0.1 M Tris, pH 7.4, 0.5% ethyl acetate, and 5 mM Tris(hydroxypropyl)phosphine, and microseeding, 1 week, 20°C, X-ray diffraction structure determination and analysis at 2.53 A resolution, molecular replacement, structure comparisons, overview |
717997 |
