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Results 1 - 4 of 4
EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.48crystal structure determination of staphopain A in complex with inhibitor E64, folding pattern
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.48inactive mutant staphopain B in complex with inhibitor staphostatin B, 21°C, vapour diffusion method with sitting drops, reservoir solution: 2 M ammonium sulfate, 5% isopropanol, 0.8% v/v 1 M guanidinium hydrochloride, 1-2 weeks, cryoprotection in buffer containing a 9:1 mixture of 2.8 M ammonium sulfate and (2R,3R)-(-)-2,3-butanediol, injected into the drops, X-ray structure determination
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.48purified recombinant mature staphopain B, sitting drop vapour diffusion method at 4°C or 21°C, equal volume of protein solution containing 15 mg/ml protein in 5mM Tris, pH 7.5, and reservoir solution containing 50 mM Bis-Tris, pH 6.5, 20% w/v PEG 4000, or 100 mM HEPES, pH 7.5, 21% w/v PEG 4000, and 10% v/v 2-propanol, are mixed, a few weeks, X-ray diffraction structure determination and analysis at 2.5-2.8 A resolution
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.48staphopain A
Results 1 - 4 of 4