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EC Number Crystallization (Commentary) Reference
Display the reaction diagram Show all sequences 3.4.21.B50sitting drop vapor diffusion method 718323
Display the reaction diagram Show all sequences 3.4.21.B50sitting-drop vapor diffusion technique. The best crystals are obtained when using 100 mM citrate (pH 5.5) and 17.5% polyethylene glycol 400 or 100 mM sodium acetate (pH 4.5), 50 mM CdCl2 and 28% polyethylene glycol 400 as reservoir solutions. Crystal structures of inactive 3-mers and active 12-mers of DegQ reveal molecular details of elements of a conserved allosteric activation cascade that defines distinct protease ON and OFF states 754420
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