EC Number   |
|---|
    3.3.2.1 | - |
| 3.3.2.1 | purified recombinant detagged AngB-ISC, sitting drop vapor diffusion, mixing of 18 mg/ml protein in 10 mM HEPES, pH 7.5, 500 mM NaCl, and 1 mM DTT, with reservoir solution containing 0.1 M Tris-HCl, pH 8.5, 2 M Li2SO4, and 2% PEG 400, at 20°C, X-ray diffraction structure determination and analysis, molecular replacement using structure VibB-ISC (PDB ID 3TB4) as the searching model, with two monomers in an asymmetric unit |
| 3.3.2.1 | purified recombinant detagged VabB-ISC, sitting drop vapor diffusion, mixing of 18 mg/ml protein in 10 mM HEPES, pH 7.5, 150 mM NaCl, and 1 mM DTT, with reservoir solution containing 10% PEG 8000, 0.1 M imidazole, and 0.2 M calcium acetate, at 20°C, X-ray diffraction structure determination and analysis, molecular replacement using the AngB-ISC dimer as search model |
| 3.3.2.1 | sitting drop vapor diffusion method, using 50 mM CaCl2, 0.1 M bis-Tris pH 6.5, 28% (w/v) polyethylene glycol monomethyl ether 550, 1% (w/v) n-octyl-beta-D-glucoside at 20°C |
| 3.3.2.1 | the crystal structure of the EntB protein is determined at a resolution of 2.3 A |
