EC Number |
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3.2.2.B5 | crystal structure of the enzyme and that of its complex with 8-oxo-guanosine at 1.0 and 1.7 A resolution, respectively, are grown by sitting drops method |
3.2.2.B5 | hanging-drop vapor diffusion at 12°C |
3.2.2.B5 | hanging-drop vapor diffusion at 12°C. A structural comparison with 8-oxoguanine DNA glycosylase 1 and archaeal GO glycosylase suggests that the C-terminal lysine of 8-oxoguanine DNA glycosylase 2 may play a key role in discriminating between guanine and 8-oxoguanine |
3.2.2.B5 | hanging-drop, vapor-diffusion method at 12°C. A single crystal of the mutant enzyme K129Q in complex with a 15-mer duplex DNA oligonucleotide containing 8-oxoG paired with C is used to collect a 2.7 A data |