EC Number   |
|---|
   3.2.2.29 | crystal structure of hTDG (catalytic domain, hTDGcat) in complex with abasic DNA, at 2.8 A resolution. The enzyme crystallizes in a 2:1 complex with DNA, one subunit bound at the abasic site, and the other at an undamaged (nonspecific) site |
| 3.2.2.29 | crystals of SUMO-1TDG complex are grown in 25% PEG 3350, 0.2 M MgCl2 and 0.1 M Tris-HCl (pH 8.5) at 20°C by using a micro-seeding technique. Crystal structure of the central region of human TDG conjugated to SUMO-1 at 2.1 A resolution |
| 3.2.2.29 | crystals of SUMO-3TDG are grown in 1.5 M sodium malonate (pH 5.0) at 20°C, by using a streaking technique. Crystal structure of the central region of TDG conjugated to SUMO-3 |
| 3.2.2.29 | enzyme bound to DNA with a non-cleavable (2'-fluoroarabino) analog of 5-formyldeoxycytidine flipped into its active site |
| 3.2.2.29 | enzyme construct TDG82-308, sitting drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 6.0 |
| 3.2.2.29 | free enzyme and in complex with DNA, sitting drop vapor diffusion method, using 30% (w/v) PEG 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 6.0 |
| 3.2.2.29 | in complex with a 28-base pair DNA containing a guanine:5-hydroxymethyluracil mismatch, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6 |
| 3.2.2.29 | in complex with duplex DNA containing either 5-carboxylcytosine or a 1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-carbonylcytosine, hanging drop vapor diffusion method, using |
| 3.2.2.29 | mutant N140A in complex with guanine:5-carboxylcytosine containing DNA, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6 |
| 3.2.2.29 | structural basis of substrate specificity |
