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EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.97purified recombinant His-tagged enzyme, X-ray diffraction structure analysis at 2.0-2.5 A resolution 708948
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.97purified recombinant isolated catalytic domain, hanging drop vapour diffusion method at 19°C, 1 ml of 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 1 ml 25% PEG 1500. Removal of the His6-tag is unnecessary for crystallization. Crystals are cryoprotected in 1 ml 33% PEG 1500 supplemented with 6% MPD, X-ray diffraction structure determination and analysis at 2.0 A resolution 706968
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