EC Number   |
|---|
   3.2.1.89 | concentrated enzyme solution crystallized in the cold |
| 3.2.1.89 | galactanase I forms a hexagonal crystal |
| 3.2.1.89 | hanging drop vapor diffusion method |
| 3.2.1.89 | hanging drops, vapour-diffusion, 30% polyethylene glycol 400, 0.2 M CaCl2, 0.1 M Na HEPES, pH 7.5, orthorhombic space group |
| 3.2.1.89 | presence of divalent cations, preferably Ca2+ |
| 3.2.1.89 | purified enzyme free or in complex with products galactobiose and galactotriose, hanging drop vapour diffusion method, 0.002-0.004 ml of protein solution, containing 20-40 mg/ml protein, are mixed with 0.002 ml reservoir solution containing 21-26% w/v PEG 1500, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement modelling |
| 3.2.1.89 | wild-type and mutant E263A in complex with methyl-beta(1-4)-galactotetraoside, grown at room temperature in hanging drops with a resolution range of 30-2.3 A. Crystals of wild-type and mutant E263A both belong to the monoclinic space group P21, containing two molecules in the asymmetric unit |
