3.2.1.85 | purified recombinant His-tagged wild-type enzyme, hanging drop vapour diffusion method, two conditions and two crystal forms: 1. mixing of 3.9-4.3 mg/ml protein with reservoir solution containing 16-18% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.5 in a 1:1 ratio, or 2. mixing of 4.3-4.5 mg/ml protein with reservoir solution containing 19-22% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.48, in a 1:1 ratio, X-ray diffraction structure determination and analysis at 1.33-1.92 A resolution, detailed three-dimensional crystal structure analysis, overview |