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EC Number Crystallization (Commentary)
Show all pathways known for 3.2.1.85Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.85hanging drop method
Show all pathways known for 3.2.1.85Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.85in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5
Show all pathways known for 3.2.1.85Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.85purified recombinant His-tagged wild-type enzyme, hanging drop vapour diffusion method, two conditions and two crystal forms: 1. mixing of 3.9-4.3 mg/ml protein with reservoir solution containing 16-18% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.5 in a 1:1 ratio, or 2. mixing of 4.3-4.5 mg/ml protein with reservoir solution containing 19-22% w/v PEG 8000, 3% v/v MPD, 0.1 M imidazole, pH 6.48, in a 1:1 ratio, X-ray diffraction structure determination and analysis at 1.33-1.92 A resolution, detailed three-dimensional crystal structure analysis, overview
Results 1 - 3 of 3