   3.2.1.170 | purified enzyme free or in complexes with glucose or glycerate, hanging drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 10 mM Tris-HCl, pH 7.5, with 0.001 ml of reservoir solution containing 20-35% v/v 2-methyl-2,4-pentanediol and 100 mM Tris-HCl, pH 5.5-6.5, for co-crystallization with glucose or glycerate, the protein solution is mixed with 10 mM ligand solution, and stored at 4°C overnight. The Tt8MGH-Glc complex crystals are grown with the same method except that 100 mM sodium citrate buffer, pH 4.8-6.0, is used instead of Tris-HCl buffer in the reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 1.77-2.10 A resolution, molecular replacement method using PDB 2Z07 as a search model, modelling |
