EC Number |
---|
3.2.1.165 | E541A mutant in complex with a natural β-1,4-D-glucosamine tetrasaccharide substrate and both E541A and D469A mutants in complex with a pNP-beta-D-glucosaminide synthetic substrate |
3.2.1.165 | hanging drop vapor diffusion method, using 14% (w/v) PEG 8000 and 0.2 M ammonium acetate in 0.1 M cacodylate buffer pH 7.0 for enzyme form Gls93-F1 or 20% (w/v) PEG 4000 and 0.2 M sodium isothiocyanate for enzyme form Gls93-F2 |
3.2.1.165 | in complex with D-glucosamine, sitting drop vapor diffusion method, using in 100 mM Tris-HCl (pH 8.5) and 20% (w/v) polyethylene glycol 1000 |
3.2.1.165 | sitting drop vapor diffusion method, using 10% (w/v) PEG 6000, 0.1 M HEPES/NaOH (pH 7.5) and 5% (w/v) 2-methyl-2,4-pentanediol |