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Results 1 - 10 of 10
EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1crystal structure analysis, PDB ID 2SNS 730207
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1crystal structure of binary Ca2+ and pdTp complexes of the D21E mutant enzyme 135039
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1crystals of the hyperstable mutant enzyme delta+PHS are grown using hanging drop vapor-diffusion methods at 4°C from a solution containing 17% 2-methyl-2,4-pentanediol, 2 M CaCl2, 3 M thymine-3',5'-diphosphate, and 25 mM potassium phosphate buffer, pH 8.0 706658
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1crystals of the I92E and I92K variant proteins are obtained at 4°C using the hanging-drop vapor-diffusion method 666025
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1E75A mutant: 4°C, hanging-drop, precipitating solution 37% (v/v) 2-methyl-2,4-pentanediol and 25 mM potassium phosphate buffer at pH 6.0, protein concentration 9.9 mg/ml before mixing with equal volume of precipitating solution. E75Q: 4°C, hanging-drop, precipitating solution 38% (v/v) 2-methyl-2,4-pentanediol and 25 mM potassium phosphate buffer at pH 6.0, starting from 14.2 mg/ml protein. Side chain of His121 is unaffected by elimination of Glu75, histidine moves closer to Glu101 in the structure with E75A. Both crystal structures suggest that the network of polar or ionizable groups connected through hydrogen bonding or charge-charge interactions is a rigid unit, incapable of reorganizing even when strongly stabilizing interactions between Glu75, His124, and Tyr93 are disrupted 699534
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1hanging drop vapor diffusion method 659163
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1LMYKGQPM, short peptide model from staphylococcal nuclease to model the conformational equilibrium between a hairpin conformation and its unfolded state (molecular dynamics simulation), in water, cubic model system, total simulation time 600 ns, starting from a polyproline II conformation, GROMOS96 force field under NVT conditions, 27°C: native and non-native hairpins are very close in free energies, interconversion can happen only through the unfolded conformation. Both folding and unfolding events display single exponential kinetics 699852
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1the V66K/P117G/H124L/S128A variant of nuclease is crystallized by the hanging drop vapor diffusion method at 4°C, 2 data sets are collected at -173.15°C, at pH 7 and 4.7, and the third is collected at 25.15°C and pH 5 702379
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1three-dimensional diffuse x-ray scattering from crystals of the enzyme 135042
Display the word mapDisplay the reaction diagram Show all sequences 3.1.31.1x-ray structure 135027
Results 1 - 10 of 10