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EC Number	Crystallization (Commentary)
3.1.3.76	crystal structure analysis
3.1.3.76	purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 12-16 mg/ml protein in 3 mM DTT, 0.1 M sodium phosphate, pH 7.4, is mixed with 0.005 ml precipitation solution containing 0.1 M Tris, pH 9.0, 30% w/v PEG 4000, and 0.2 M Li2SO4, versus 1 ml reservoir of precipitatin solution, 4°C, 10 days, X-ray diffraction structure determination and analysis at 2.6 A resolution, modeling

EC Number

Crystallization (Commentary)

3.1.3.76

crystal structure analysis

3.1.3.76

purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 12-16 mg/ml protein in 3 mM DTT, 0.1 M sodium phosphate, pH 7.4, is mixed with 0.005 ml precipitation solution containing 0.1 M Tris, pH 9.0, 30% w/v PEG 4000, and 0.2 M Li2SO4, versus 1 ml reservoir of precipitatin solution, 4°C, 10 days, X-ray diffraction structure determination and analysis at 2.6 A resolution, modeling

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Release 2023.1 (January 2023)