3.1.27.8 | purified recombinant His-tagged truncated enzyme mutant hEndoV-SF, sitting drop vapour diffusion method, mixing of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.5, 300 mM NaCl, is mixed with 19% PEG 3350, 0.2 M sodium formate, 0.1 M Tris-HCl pH 7.5, or with 1.4 M ammonium sulfate, 0.2 M sodium acetate, 0.1 M Tris-HCl pH 7.5, as crystallization solution, method screening and optimization, 25°C, 2-3 days, X-ray diffraction structure determination and analysis at 2.3 a resolution |