3.1.2.25 | hanging drop vapor diffusion method. Crystals of wild type enzyme and mutants N37A, D52A, and T68A are obtained in conditions composed of 200 mM ammonium tartrate dibasic, 20% (w/v) PEG 3350, 100 mM Tris, pH 7.5; 200 mM lithium sulfate, 100 mM Tris-HCl, 30% (w/v) PEG 4000, 200 mM magnesium chloride, PEG 400, 100 mM HEPES, pH 7.5; 200 mM magnesium chloride, 14% (w/v) PEG 3350, 100 mM Tris, pH 6.0, respectively |