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EC Number	Crystallization (Commentary)
3.1.13.5	1.6 A resolution crystal structure. Hanging-drop technique was used for subsequent crystal optimizations. Each drop contains equal volumes of protein and reservoir solution. RNase D protein crystallizes in the presence of 2.02.5 M ammonium sulfate and various buffer conditions. The crystals used here are grown with a reservoir containing 2.22.3 sulfate and 0.1 M HEPES (pH 7.0). Crystals of RNase D usually appeared overnight under these conditions. Crystals grow to full size in 27 days, with a maximum size of over 1 mm in each dimension. Zn2+ -containing crystals are grown with 1 mM ZnSO4 included in the reservoir prior to drop setup. Mercury(II) derivatives are prepared by soaking RNase D crystals (without Zn2+) overnight in the mother liquor containing 5 mM HgSO4

EC Number

Crystallization (Commentary)

3.1.13.5

1.6 A resolution crystal structure. Hanging-drop technique was used for subsequent crystal optimizations. Each drop contains equal volumes of protein and reservoir solution. RNase D protein crystallizes in the presence of 2.02.5 M ammonium sulfate and various buffer conditions. The crystals used here are grown with a reservoir containing 2.22.3 sulfate and 0.1 M HEPES (pH 7.0). Crystals of RNase D usually appeared overnight under these conditions. Crystals grow to full size in 27 days, with a maximum size of over 1 mm in each dimension. Zn2+ -containing crystals are grown with 1 mM ZnSO4 included in the reservoir prior to drop setup. Mercury(II) derivatives are prepared by soaking RNase D crystals (without Zn2+) overnight in the mother liquor containing 5 mM HgSO4

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Release 2023.1 (January 2023)