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EC Number Crystallization (Commentary) Reference
Display the reaction diagram Show all sequences 3.1.1.85hanging drop vapor diffusion method, using 1.2 M sodium citrate trihydrate and 0.1 M Tris-HCl (pH 8.0), at 21°C 652416
Display the reaction diagram Show all sequences 3.1.1.85sitting drop vapor diffusion method at 16 °C. The crystal structure is determined at at 1.26 A. The BioG structure is similar to the BioH structure (from Escherichia coli) and is composed of an alpha-helical lid domain and a core domain that contains a central seven-stranded beta-pleated sheet. However, four of the six alpha-helices that flank both sides of the BioH core beta-sheet are replaced with long loops in BioG, thus forming an unusual alpha/beta-hydrolase fold 749909
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