EC Number |
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3.1.1.31 | by the hanging-drop method, 6PGL in complex with two ligands, 6-phosphogluconic acid and citrate, at 2.2 A and 2.0 A resolution, respectively. Structures of the two complexes and of the apo form are almost identical. Both complexes belong to space group P21 and have two molecules in the asymmetric unit, related by a pure translation. PGL in complex with 6-phosphogluconic acid has unit cell parameters of a = 49.66, b = 87, c= 64.91 A and beta = 96.43. PGL in complex with citrate has unit cell parameters of a = 49.46, b = 87.21, c= 64.79 A and beta = 96.53 |
3.1.1.31 | hanging drop vapour diffusion method, using 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, and 0.2 M ammonium acetate |
3.1.1.31 | hanging-drop method, equilibrated against a reservoir solution of 100 mM Hepes (pH 7.5), 30% (v/v) PEG MME 2000, 50 mM sodium acetate |
3.1.1.31 | homology modeling of structure |