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EC Number Crystallization (Commentary)
Display the reaction diagram Show all sequences 3.1.1.27by the sitting-drop vapour-diffusion method, at 4°C. Free enzyme to 2.0 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 77.93, b = 38.88, c = 81.60 A, beta = 117.33. The 5-pyridoxolactone-bound enzyme to 1.9 A resolution, belongs to the monoclinic space group C2, with unit-cell parameters a = 86.24, b = 39.35, c = 82.68 A, beta = 118.02
Display the reaction diagram Show all sequences 3.1.1.27native and SeMet-labelled protein in complex with 5-pyridoxolactone, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 50 mM ammonium citrate, pH 5.1
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