EC Number   |
|---|
   3.1.1.11 | complex between enzyme and PMEI, vapor diffusion technique |
| 3.1.1.11 | hanging drop vapor diffusion method |
| 3.1.1.11 | purified enzyme in deglycosylated and Asn-linked N-acetylglucosamine-stub forms, hanging drop vapor diffusion method, drops of 0.002 ml from protein and reservoir solutions in the ratios of 1:1 and 2:3, the latter containing 1.9 M (NH4)2SO4, 100 mM sodium acetate, pH 3.6, (EndoHf-deglycosylated Ani-PME2), or 1.8 M (NH4)2SO4, 100 mM sodium acetate, pH 4.1 (PNGaseF-deglycosylated Ani-PME2), 21°C, 1 week, method optimization, X-ray diffraction structure determination and analysis at 1.75-1.80 A resolution, molecular replacement using the structure of PMEs from Solanum lycopersicum (PDB code 1xg2) and Daucus carota (PDB code 1gq8) as search models |
| 3.1.1.11 | sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M citric acid pH 3.5 |
| 3.1.1.11 | the inactive D178A PME mutant in complex with specifically methylated hexagalacturonates, 3 mg/ml protein, crystallization solutions contain 0.1 M MES, pH 6.5, 10% dioxane and 1.6 M ammonium sulfate, and dilutions of that crystallisation buffer with H2O, or 0.1 M MES, pH 6.5, and 12% w/v PEG 20000, X-ray diffraction structure determination and anaylsis at 1.7-1.9 A resolution |
