EC Number   |
Reference |
|---|
    2.8.1.7 | - |
661090 |
| 2.8.1.7 | C327S mutant in complex with pyridoxal 5'-phosphate and in the absence of L-cysteine, hanging drop vapor diffusion method, using 20% (w/v) PEG 3350, 0.1 M sodium acetate, pH 5.5, and 0.2 M ammonium citrate |
726296 |
| 2.8.1.7 | comparison of Helicobacter pylori isoform NifS (type I enzyme) and Bacillus subtilis isoform SufS (type II enzyme). For both, the thiol group of the PLP-L-cysteine external aldimine is stabilized by the conserved histidine adjacent to PLP through a polar interaction, orientating the thiol group for subsequent nucleophilic attack by a conserved cysteine residue on the catalytic loop in the state of PLP-L-cysteine ketimine, which is formed from the PLP-L-cysteine aldimine. In the type I enzyme, conformational and topological change of the loop is necessary for nucleophilic attack by the cysteine. The loop in type II cysteine desulfurase enzymes shows no large conformational change. It might orient the thiol group of the catalytic cysteine for nucleophilic attack toward PLP-L-cysteine |
761068 |
| 2.8.1.7 | CsdA and CsdACsdE complex, hanging drop vapor diffusion method, using 20-30% (w/v) PEG 8000, 0.05 M potassium di-hydrogen phosphate, and 0.1 M MES, pH 6.5 (or pH 5.5), or 20-30% (w/v) PEG 8000, 0.05 M sodium di-hydrogen phosphate, and 0.1 M MES, pH 6.5 (or 0.1 M Tris, pH 8.5) |
738590 |
| 2.8.1.7 | hanging drop vapor diffusion method, complexed with L-propargylglycine |
645630 |
| 2.8.1.7 | hanging drop vapor diffusion method, using 0.2 M calcium acetate, 0.1 sodium cacodylate pH 6.5, 40% (v/v) PEG 300 |
724811 |
| 2.8.1.7 | homodimer in complex with pyrodoxal 5'-phosphate, alanine, and Cys361-persulfide, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 50% (w/v) PEG 400 |
739453 |
| 2.8.1.7 | homology modeling of structure |
760523 |
| 2.8.1.7 | microbatch-under-oil method, using 20% (w/v) PEG 3000, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5 |
737375 |
| 2.8.1.7 | modeling of structure |
672753 |
