EC Number   |
|---|
  2.8.1.15 | in complex with TtuB sulfur carrier protein, to 2.5 A resolution |
| 2.8.1.15 | sitting drop vapor diffusion method at 208C, using 1 microl of sample solution mixed with an equal volume of reservoir solution, composed of 100 mM BicineNaOH buffer (pH 9.0) containing 200 mM NaCl, 12% (w/v) polyethylene glycol(PEG) 3350, and 010% (v/v) N,N-dimethylformamide. The native crystal belongs to the space group P43212, with unit cell parameters of a 5 b 5 70.1 Angstrom and c 5 128.6 Angstrom |
| 2.8.1.15 | structure of the TtuA-TtuB complex, at a resolution of 2.5 A, showing the S transfer of TtuB to tRNA using its C-terminal thiocarboxylate group. The active site of TtuA is connected to the outside by two channels, one occupied by TtuB and the other used for tRNA binding |
| 2.8.1.15 | to 2.1 A resolution. TtuA forms a homodimer, and each subunit contains a catalytic domain and unique N- and C-terminal zinc fingers. Three conserved cysteine residues are clustered in the putative catalytic site |
| 2.8.1.15 | vapour-diffusion method at 293 K under anaerobic conditions, 2-thioribothymidinesynthetic complex TtuATtuB |
