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Results 1 - 3 of 3
EC Number Crystallization (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.83purified recombinant detagged AGX1A229T in complex with UDP-GlcNAc, X-ray diffraction structure determination and analysis at 1.7 A resolution, molecular replacement using the published AGX1 structure (PDB ID 1JV1) as a search model
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.83purified recombinant enzyme mutant Y97N in complex with UTP or N-acetyl-alpha-D-glucosamine 1-phosphate, sitting drop vapor diffusion method, mixing of 11 mg/ml protein with ligands 5 mM UDP-GlcNAc and 10 mM (NH4)2SO4 (to form the complex with UDP-GlcNAc) or 5 mM UTP and 10 mM (NH4)2SO4 (to form the complex with UTP), and with cyrstallization solution containing 20% w/v PEG 3350 and 0.2 M potassium citrate tribasic monohydrate, X-ray diffraction structure determination and analysis at 2.91 and 2.09 A resolution, respectively
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.83vapor diffusion technique, PEG600, 20°C, pH5.7
Results 1 - 3 of 3