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Results 1 - 8 of 8
EC Number Crystallization (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72cocrystal structures of the enzyme complexed with both a tRNA mimic and nucleoside triphosphates under catalytically active conditions. The structures suggest that adenosine 5'-monophosphate is incorporated onto the A76 position of the tRNA via a carboxylate-assisted, one-metal-ion mechanism with aspartate 110 functioning as a general base. The discrimination against incorporation of cytidine 5'-triphosphate at position 76 arises from improper placement of the a phosphate of the incoming CTP, which results from the interaction of C with arginine 224 and prevents the nucleophilic attack by the 3' hydroxyl group of cytidine75 710564
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72cocrystallisation of enzyme complexes with nine distinct tRNA minihelices. All of the binary and ternary complex crystals belong to the space group P4(3)2(1)2, and contain one complex molecule in the asymmetric unit. Crystal structures are solved at 2.5 to 3.05 A resolutions by molecular replacement 722113
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72crystal structure analysis 721997
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72crystal structures of the CCA-adding enzyme, and its complexes with CTP and ATP at 2.0, 2.0 and 2.7 A resolutions, respectively 722111
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72hanging-drop vapour diffusion method at 4°C, crystal structure of the enzyme in complex with tRNA 660002
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72in complex with a human MenBeta minihelix. The unstable minihelix is bound between the enzyme’s catalytic center, comprised of the head and neck domains, and its tail domain. The minihelix perfectly mimics full-length tRNA with its acceptor and TPsiC stems folding into a continuous A-type RNA helix. The TPsiC loop is in the same conformation as in full-length tRNA 738024
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72modeling of a loop sequence inserted into the structure of human CCA-adding enzyme based on pdb-entry 1OU5. The conserved loop residue R105 forms a salt bridge to the first residue E164 of the amino acid template EDxxR in motif D 710150
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.72purified recombinant monomeric native enzyme or enzyme in complex with thimerosal, sitting drop vapor diffusion method, mixing of 0.003-0.005 ml of protein solution with 0.001 ml of reservoir solution containing 100 mM sodium citrate, pH 5.6, 2.2 M ammonium sulfate, and equilibration against 0.4 ml of reservoir solution, 3 days to 3 weeks, for ligand bound enzyme crystals soaking in reservoir solution containing 0.5 mM thimerosal for 45 min, 18°C, X-ray diffraction structure determination and analysis at 3.4-3.7 A resolution, single isomorphous replacement 643052
Results 1 - 8 of 8