  2.7.7.71 | purified recombinant His6-tagged wild-type and SeMet-labeled enzymes with bound GMPPNP and Mg2+, hanging drop vapor diffusion method, mixing of 800 nl of 12 mg/ml protein in 20 mM HEPES, pH 7.5, with 800 nl of reservoir solution containing 0.1 M HEPES, pH 7.4, and 1.8 M ammonium sulfate for the wild-type, and 0.1 M HEPES, pH 7.0, and 2.2 M ammonium sulfate for the labeled enzyme, equilibration against 0.20 ml reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 1.9-2.0 A resolution, modeling |
| 2.7.7.71 | purified recombinant wild-type His6-tagged enzyme with bound GMPPNP and Mg2+, hanging drop vapor diffusion method, from 0.05 M HEPES, pH 7.5, 1.2 M sodium citrate tribasic dehydrate, and 0.2 M MgCl2, room temperature, method optimization, X-ray diffraction structure determination and analysis at 1.55 A resolution, molecular replacement using the coordinates of native YpHddC (PDB ID 5XHW) as a search model |
